| distance | reference number | name | latitude | longitude |
| 21.3 | 602 | Cocohatchee River, U.S. 41 bridge, Florida | 26.2817 | -81.8017 |
| 22.2 | 3340 | Wiggins Pass, Cocohatchee River, Florida | 26.29 | -81.8183 |
| 22.8 | 1943 | Naples, Naples Bay, north end, Florida | 26.1367 | -81.7883 |
| 23.8 | 603 | Coconut Point, Estero Bay, Florida | 26.4 | -81.8333 |
| 24.0 | 1942 | Naples, Gulf Of Mexico, Florida | 26.13 | -81.8067 |
| 24.2 | 2466 | Pumpkin Bay, Florida | 25.9167 | -81.55 |
| 24.3 | 1647 | Little Hickory Island, Estero Bay, Florida | 26.35 | -81.85 |
| 25.6 | 935 | Estero Bay, Estero River, Florida | 26.43 | -81.8567 |
| 26.2 | 1755 | Marco, Big Marco River, Florida | 25.9717 | -81.7283 |
| 26.7 | 465 | Carlos Point, Estero Bay, Florida | 26.4 | -81.8833 |
| 26.8 | 632 | Coon Key, Florida | 25.9 | -81.6333 |
| 27.2 | 946 | Everglades City, Barron River, Florida | 25.8583 | -81.3867 |
| 28.9 | 2593 | Round Key, Florida | 25.8333 | -81.5333 |
| 29.2 | 485 | Caxambas Pass, Marco Island, Florida | 25.9083 | -81.7283 |
| 30.1 | 560 | Chokoloskee, Florida | 25.8133 | -81.3633 |
| 30.4 | 1379 | Indian Key, Florida | 25.8 | -81.4667 |
| 30.8 | 450 | Cape Romano, Florida | 25.85 | -81.6833 |
| 31.3 | 1777 | Matanzas Pass (fixed bridge) Estero Island, Florida | 26.45 | -81.95 |
| 32.6 | 1028 | Fort Myers, Caloosahatchee River, Florida | 26.6467 | -81.8717 |
| 32.6 | 1029 | Fort Myers, Florida | 26.6467 | -81.8717 |
| 33.0 | 429 | Cape Coral Bridge, Florida | 26.5667 | -81.9333 |
| 33.6 | 1389 | Iona Shores, Caloosahatchee River, Florida | 26.5167 | -81.9667 |
| 35.2 | 2333 | Point Ybel, San Carlos Bay Entrance, Florida | 26.45 | -82.0167 |
| 35.7 | 2472 | Punta Rassa, San Carlos Bay, Florida | 26.4833 | -82.0167 |
| 38.7 | 525 | Chatham River entrance, Florida | 25.6833 | -81.2833 |