| distance | reference number | name | latitude | longitude |
| 2.9 | 2754 | Shark River entrance, Florida | 25.35 | -81.1333 |
| 7.5 | 3333 | Whitewater Bay, Florida | 25.3167 | -81.0333 |
| 12.3 | 452 | Cape Sable, East Cape, Florida | 25.1167 | -81.0833 |
| 15.0 | 1697 | Lostmans River entrance, Florida | 25.55 | -81.2167 |
| 17.2 | 996 | Flamingo, Florida Bay, Florida | 25.1417 | -80.9233 |
| 18.6 | 1698 | Lostmans River, Onion Key, Florida | 25.6167 | -81.1333 |
| 23.8 | 525 | Chatham River entrance, Florida | 25.6833 | -81.2833 |
| 32.2 | 852 | East Bahía Honda Key, south end, Florida Bay, Flori | 24.775 | -81.2267 |
| 32.4 | 3294 | West Bahía Honda Key, Florida | 24.78 | -81.2717 |
| 32.7 | 560 | Chokoloskee, Florida | 25.8133 | -81.3633 |
| 33.3 | 1326 | Horseshoe Keys, south end, Florida | 24.7667 | -81.2833 |
| 34.0 | 1431 | Johnson Keys, north end, Florida | 24.7667 | -81.3233 |
| 34.1 | 600 | Cocoanut Key, Florida Bay, Florida | 24.745 | -81.2367 |
| 34.4 | 2894 | Spanish Banks, Little Spanish Key, Florida | 24.775 | -81.37 |
| 34.7 | 251 | Big Spanish Key, Florida | 24.7883 | -81.4117 |
| 34.7 | 1141 | Grassy Key (North), Florida Bay, Florida | 24.7717 | -80.94 |
| 34.8 | 3112 | Toms Harbor Channel, Hwy. 1 bridge, Florida | 24.7767 | -80.9233 |
| 34.9 | 1379 | Indian Key, Florida | 25.8 | -81.4667 |
| 34.9 | 3113 | Toms Harbor Cut, Florida | 24.7833 | -80.9067 |
| 34.9 | 1432 | Johnson Keys, south end, Florida | 24.7433 | -81.3 |
| 35.0 | 1648 | Little Pine Key, north end, Florida | 24.75 | -81.3283 |
| 35.1 | 622 | Conch Key, eastern end, Florida | 24.7917 | -80.8833 |
| 35.2 | 248 | Big Spanish Channel, Crawl Key, Florida | 24.7567 | -81.3583 |
| 35.3 | 1142 | Grassy Key, south side, Hawk Channel, Florida | 24.755 | -80.9583 |
| 35.5 | 966 | Fat Deer Key, Florida Bay, Florida | 24.7333 | -81.0183 |